|
KMID : 0624620230560030190
|
|
BMB Reports
2023 Volume.56 No. 3 p.190 ~ p.195
|
|
|
MicroRNA super-resolution imaging in blood for Alzheimer’s disease
|
|
Lee Mi-Rae
Woo Ji-Won
Kim Sang-Tae
Moon Min-Ho
Kim Sang-Yun
Cho Han-Na
Kim Su-Jin
Kim Han-Kyeol
Park Jeong-Yoon
|
|
|
Abstract
|
|
|
|
We propose a novel blood biomarker detection method that uses miRNA super-resolution imaging to enable the early diagnosis of Alzheimer’s disease (AD). Here, we report a singlemolecule detection method for visualizing disease-specific miRNA in tissue from an AD mice model, and peripheral blood mononuclear cells (PBMCs) from AD patients. Using optimized Magnified Analysis of Proteome (MAPs), we confirmed that five miRNAs contribute to neurodegenerative disease in the brain hippocampi of 5XFAD and wild-type mice. We also assessed PBMCs isolated from the whole blood of AD patients and a healthy control group, and subsequently analyzed those samples using miRNA super-resolution imaging. We detected more miR-200a-3p expression in the cornu ammonis 1 and dentate gyrus regions of 3 month-old 5XFAD mice than in wild-type mice. Additionally, miRNA super-resolution imaging of blood provides AD diagnosis platform for studying miRNA regulation inside cells at the single molecule level. Our results present a potential liquid biopsy method that could improve the diagnosis of early stage AD and other diseases.
|
|
|
KEYWORD
|
|
|
Alzheimer’s disease, Biomarker, Liquid biopsy, Magnified analysis of proteome, microRNA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|